SRA

Locust plagues are one of the main biological disasters occurring in grassland, posing aserious threat to agriculture, animal husbandry, and grassland ecological security. Matrine is a highlypotent plant pesticide that, when applied artificially, may efficiently manage the number of locustsin their natural habitat. In order to determine the specific genes and proteins that are affected bymatrine and play a role in regulating metabolism in the locust species Oedaleus asiaticus, weconducted RNA-seq, proteomic sequencing, and bioinformatics analyses on third-instar nymphs.These nymphs were divided into two groups: one group was grown under normal conditions, whilethe other group was treated with matrine. The purpose of this investigation was to gain insight intothe molecular mechanisms underlying matrine resistance. Genes and proteins that exhibiteddifferential expression were identified and subjected to analysis using bioinformatics software.Additionally, enrichment studies based on Gene Ontology (GO) and the Kyoto Encyclopedia ofGenes and Genomes (KEGG) were performed. The findings demonstrated that matrine exertedcontrol of the growth of O. asiaticus via modulating the synthesis of chitin and hormones. Increasedexpression of detoxifying enzymes was observed, which may be related to matrine detoxification.These findings provide a basis for better comprehension of the molecular mechanism behind theregulation of development mediated by matrine in Asian locust hoppers.